TbIRK is a signature sequence free potassium channel from Trypanosoma brucei locating to acidocalcisomes
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چکیده
منابع مشابه
A novel C-terminal kinesin is essential for maintaining functional acidocalcisomes in Trypanosoma brucei.
Kinesins are cytoskeletal motor proteins that play roles in a variety of fundamental cellular processes including cell division and the anterograde transport of vesicles and organelles. We purified, cloned, and functionally characterized in Trypanosoma brucei a new member of the C-terminal kinesin family, TbKIFC1. Kinetic constants of the recombinant motor domain of TbKIFC1 were estimated at 0....
متن کاملCharacterization of a vacuolar pyrophosphatase in Trypanosoma brucei and its localization to acidocalcisomes.
Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized procyclic trypomastigotes of Trypanosoma brucei, as measured by acridine orange uptake. The proton gradient generated by pyrophosphate was collapsed by addition of nigericin or NH(4)Cl. Pyrophosphate-driven proton translocation was stimulated by potassium ions and inhibited by KF, by the pyrophos...
متن کاملTrypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes
Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BS...
متن کاملTrypanosoma brucei brucei
Protein synthesis in Trypanosoma brucei brucei was rapidly inhibited during polyamine depletion by DL-adifluoromethylornithine (DFMO) in vitro and in vivo. [3H]Leucine incorporation was depressed 30-40 % by 24 h and 80-90 % by 48 h of DFMO treatment. Concomitantly there was an apparent decrease in the synthesis of the variant-specific glycoprotein (VSG) in DFMO-treated trypanosomes, as measured...
متن کاملSequence of a tRNA gene cluster in Trypanosoma brucei.
A recent analysis of tRNA genes in Trypanosoma brucei has revealed an unusual genomic organisation where several genes encoding tRNAs and other small RNAs are very closely clustered (1). Genes encoding tRNA* (anticodon CUU) and tRNA^ (ACG) were found tightly linked to genes encoding a U snRNA (U RNA B, ref. 2) and a 275 nt RNA X of unknown function. At a separate genomic locus genes encoding tR...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-00752-1